Real-time PCR plays a foundational role in gene expression studies, disease analysis, and molecular diagnostics. However, success depends on precise reaction setup, optimized assays, and accurate ...

In a recent study published in Lancet Microbe, researchers compare the potential of real-time reverse transcriptase-polymerase chain reaction (RT-PCR) genotyping assays with whole-genome sequencing ...

How it Works: Real Time PCR Brendan Maher The instrumentation is basic: a thermal cycler for amplification, a light source for excitation of fluorescent probes (see chemistries below), a camera for ...

Real-time PCR (qPCR) analysis has become the method of choice for quantitating DNA in a wide variety of applications. The availability of commercial kits has made the technique easy to perform, ...

Real-time PCR has gained significant acceptance by high-complexity clinical laboratories developing tests for a variety of applications. Sensitivity, automation, and turnaround time have made PCR ...

It’s best to test for RSV soon after your symptoms start. That’s because you’ll likely have the most virus in your system then. As you get better, the amount of virus will go down. (Photo Credit: ...

Arthropod-borne diseases such as Dengue, Chikungunya, Malaria (caused by Plasmodium spp.), and Zika are endemic and re-emerging infections in tropical and subtropical regions. During heavy monsoon ...